Characterization of humoral immune Responses against capsid protein p24 and transmembrane glycoprotein gp41 of human immunodeficiency virus type 1 in China

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Abstract

The objective of this study was to extend our previous research and to further characterize the humoral immune responses against HIV-1 p24, gp41 and the specific peptides carrying the immunodominant epitopes (IDEs) that react with human serum samples from HIV-1-infected individuals in China. We found that the majority (90.45%, 180/199) of the samples did not react with any of the three HIV-1 p24 peptides carrying IDEs, but did react with the recombinant full-length p24, suggesting that these samples tested in China were primarily directed against the conformational epitopes of HIV-1 p24. In contrast, 84.54% (164/194) of the samples reacted with at least one HIV-1 linear gp41 peptide, in particular the gp41-p1 peptide (amino acids 560-616). Both recently and long-term HIV-1-infected individuals displayed similar humoral immune responses against the recombinant gp41. However, samples from long-term HIV-1-infected subjects but not from recently infected subjects, showed a very strong reaction against the gp41-p1 peptide. The different response patterns observed for the two groups against the gp41 and the peptide gp41-p1 were statistically significant (P<0.01, Chi-square test). These results have direct relevance and importance for design of improved HIV-1 p24 detection assays and the gp41-based immunoassay that can be used to reliably distinguish recent and long-term HIV-1 infection.

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Li, X., Wu, Y., Ren, X., Deng, S., Hu, G., Yu, S., & Tang, S. (2016). Characterization of humoral immune Responses against capsid protein p24 and transmembrane glycoprotein gp41 of human immunodeficiency virus type 1 in China. PLoS ONE, 11(11). https://doi.org/10.1371/journal.pone.0165874

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