Culture of explanted rat embryos in rotating tubes

Abstract

It appears that roller tubes are as effective as more elaborate culture methods for maintaining the growth of embryos, provided tubes of adequate size are used for the particular stage of embryonic development. Roller tubes are inconvenient for continuous or frequent observation of the embryos during culture but, where the main object is to assess the final result of a treatment (e.g. a test substance added to the culture medium) on embryonic development, the simplicity of the method has much to recommend it.