ANTIOXIDANT PROPERTIES AND QUANTIFICATION OF PHENOLIC AND FLAVONOID COMPOUNDS IN Alpinia purpurata (Viell.) K. Schum ETHANOL EXTRACT

Abstract

Endophytic fungi are organisms that live on plant tissues without causing negative effects which the endophyte can produce secondary metabolites probably same with host plant. Research ever done before is isolate the active trace of ethyl acetate extracts of culture media endophytic fungi Athelia rolfsii Strain orchid from the stems of red betel shown to have cytotoxic activity against breast cancer cell line T47D with IC 50 of 9.2 mg/mL and in vero cells with IC 50 109 mg/mL. This study aims to determine the effect of variations in the fermentation conditions (type of media, carbon source, nitrogen source, temperature, pH and salinity) on the production of biomass of endophytic fungi Atheliarolfsii Strain orchid. Misellium dry weights calculated as the product of the dry weight of the biomass and the supernatant liquid-liquid extraction results from media and ethyl acetate metabolites is calculated as the total. Results of variation in fermentation conditions, the optimum conditions obtained are: biomass production by supplementation with glucose, yeast, SDB, a temperature of 29 °C, pH 5.