Characterization of reactive oxygen/ nitrogen species produced in PBS and DMEM by air DBD plasma treatments

Abstract

Atmospheric pressure air dielectric barrier discharges were applied to phosphate-buffered saline and complete Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum to investigate their oxidative chemical modifications induced in liquids that are relevant for cell culture experiments in the field of Plasma Medicine. The study mainly identified long-living reactive species. Hydrogen peroxide, superoxide anion radicals, and nitrite and nitrate ions were detected in DMEM. The density of the reactive species was correlated with the energy dose delivered to the liquids by the discharges.