Relation of photochemical reflectance indices based on differentwavelengths to the parameters of light reactions in photosystems I and II in pea plants

Abstract

Measurement and analysis of the numerous reflectance indices of plants is an effective approach for the remote sensing of plant physiological processes in agriculture and ecological monitoring. A photochemical reflectance index (PRI) plays an important role in this kind of remote sensing because it can be related to early changes in photosynthetic processes under the action of stressors (excess light, changes in temperature, drought, etc.). In particular, we previously showed that light-induced changes in PRIs could be strongly related to the energy-dependent component of the non-photochemical quenching in photosystem II. The aim of the present work was to undertake comparative analysis of the efficiency of using light-induced changes in PRIs (DPRIs) based on different wavelengths for the estimation of the parameters of photosynthetic light reactions (including the parameters of photosystem I). Pea plants were used in the investigation; the photosynthetic parameters were measured using the pulse-amplitude-modulated (PAM) fluorometer Dual-PAM-100 and the intensities of the reflected light were measured using the spectrometer S100. The DPRIs were calculated as DPRI(band,570), where the band was 531 nm for the typical PRI and 515, 525, 535, 545, or 555 nm for modified PRIs; 570 nm was the reference wavelength for all PRIs. There were several important results: (1) DPRI(525,570), DPRI(531,570), DPRI(535,570), and DPRI(545,570) could be used for estimation of most of the photosynthetic parameters under light only or under dark only conditions. (2) The combination of dark and light conditions decreased the efficiency of DPRIs for the estimation of the photosynthetic parameters; DPRI(535,570) and DPRI(545,570) had maximal efficiency under these conditions. (3) DPRI(515,570) and DPRI(525,570) mainly included the slow-relaxing component of PRI; in contrast, DPRI(531,570), DPRI(535,570), DPRI(545,570), and DPRI(555,570) mainly included the fast-relaxing component of PRI. These components were probably caused by different mechanisms.