Impact of Anthropometric and Genetic Factors on Plasma SIRT1 Level in Men with Essential Hypertension and Heart Failure

Abstract

Introduction: Sirtuin 1 (SIRT1) emerges as a promising biomarker for heart remodeling in th context of essential arterial hypertension (EAH). Additionally, the levels of certain plasma peptide might signal myocardial fibrosis and the progression of heart failure (HF) in hypertensive patients Despite this potential, conflicting data exist regarding the marker's diagnostic value in HF patients This study aims to explore the impact of anthropometric factors on plasma SIRT1 levels in men di agnosed with EAH and HF, considering the rs7069102 single nucleotide polymorphism (SNP) in th SIRT1 gene. Methods: The study included an examination of 190 Ukrainian men aged betwee 40 and 65. The participants were divided into two groups: a control group, consisting of 70 indi viduals without cardiovascular disease (CVD), and a study group comprising 120 men with EAH 60 of whom displayed signs of HF. Plasma SIRT1 levels were quantified using an enzyme-linked im munosorbent assay (ELISA), while the rs7069102 C/G polymorphism in the SIRT1 gene was detecte through allele-specific polymerase chain reaction (PCR). The research employed various statistica methods, including correlation analysis, T-test, Mann-Whitney U test, one-way ANOVA, and analysi of contingency tables for data analysis. Results: The investigation revealed that men suffering from EAH and HF exhibited significantly reduced plasma SIRT1 levels (1.550 ± 0.084 ng/ml) compare to those with EAH but without HF (3.271 ± 0.238 ng/ml, p < 0.001). Notably, hypertensive me with concurrent HF and obesity or those with an early onset of hypertension showed even lowe plasma SIRT1 concentrations. Interestingly, the analysis found no significant difference in plasm SIRT1 levels among individuals in the control group and EAH patients without HF across differen rs7069102 C/G SNP variants. However, among hypertensive men with HF, individuals with the GG genotype displayed considerably lower plasma peptide levels compared to those with either th CC or CG genotype (1.390 ± 0.092 ng/ml vs. 1.744 ± 0.126 ng/ml, p = 0.032). Conclusion: Thi study highlights a significant association between reduced plasma SIRT1 levels and heart failur in male patients with EAH. Factors contributing to decreased plasma peptide levels include obe sity, early onset of hypertension, and possessing the GG variant of the rs7069102 SNP in the SIRT gene. These findings underscore the potential of SIRT1 as a marker for HF and may guide futur therapeutic strategies.