Strength in numbers: Quantitative single-molecule RNA detection assays

Abstract

Gene expression is a fundamental process that underlies development, homeostasis, and behavior of organisms. The fact that it relies on nucleic acid intermediates, which can specifically interact with complementary probes, provides an excellent opportunity for studying the multiple steps-transcription, RNA processing, transport, translation, degradation, and so forth-through which gene function manifests. Over the past three decades, the toolbox of nucleic acid science has expanded tremendously, making high-precision in situ detection of DNA and RNA possible. This has revealed that many-probably the vast majority of-transcripts are distributed within the cytoplasm or the nucleus in a nonrandom fashion. With the development of microscopy techniques we have learned not only about the qualitative localization of these molecules but also about their absolute numbers with great precision. Single-molecule techniques for nucleic acid detection have been transforming our views of biology with elementary power: cells are not average members of their population but are highly distinct individuals with greatly and suddenly changing gene expression, and this behavior of theirs can be measured, modeled, and thus predicted and, finally, comprehended.