Molecular identification of different actinomycetes isolated from East Black Sea region plateau soil by 16S rDNA gene sequencing

Abstract

In order to obtain different actinomycetes isolates from the East Black Sea Region plateau soil, an isolation study was carried out by using sucrose gradient method and different growth media. A molecular taxonomic method, 16S rDNA gene sequencing, was applied to obtain different actinomycetes isolates from East Black Sea region plateau soil. Phylogenetic dendrograms based on 16S rDNA nucleotide sequences were constructed by using neighbour-joining algorithm. In the light of phylogenetic analyses, it was determined that out of 15 organisms, two belong to Actinomadura, three Kribbella, three Nocardia, six Micromonospora and an organism of Microbacterium. Soil isolates obtained from this study contribute to taxonomy of actinomycetes and also many species will emerge.   Key words: Actinomadura, Kribbella, Microbacterium, Micromonospora, Nocardia, 16S rDNA gene sequencing.