Seduhan Bubuk Kopi Robusta Spray Dryed Meningkatkan Viabilitas Monosit yang Dipapar Eksotoksin Streptococcus mutans (In Vitro)

Abstract

Monocytes response against bacterial exotoxins can cause cell damage, due to the production of antimicrobial compounds such as oxidants and free radicals. Spray dryed robusta coffee brewed contained antioxidants that was alleged to be able counteract oxidants and free radicals leading to prevent cell damage. This research aimed to prove the effect of spray dryed robusta coffee brew (SDRCB) to increase monocytes viability after being exposed by exotoxins of Streptococcus mutans (in vitro). This in vitro experimental study used the post test only control group design. The object of study was human peripheral blood monocytes, isolated by means of gradient centrifugation method. Monocyte viability was analyzed by trypan blue assay. The parameter was the percentage viable monocytes. Data were analyzed using Anova and LSD. Monocytes incubated with SDRCB showed a significant (p<05) higher viability (96.95%) than monocytes without SDRCB (94,27%). Spray dryed robusta coffee brew proved to be able increase viability of monocytes after S.mutans exposure (in vitro).