Preparation of Blueberry Anthocyanin Nanoemulsions and Their Stability and Skin Safety Evaluation

Abstract

Blueberry anthocyanins have strong antioxidant activity, but their instability and low bioavailability limit their use. Nanoemulsions are new type of food and drug carrier with stable thermodynamic properties. Therefore, anthocyanins will be encapsulated with nanoemulsions to improve their stability and application value. In this study, the best surfactants, oil phases and cosurfactants for the preparation of blueberry anthocyanin nanoemulsions were screened by pseudo-ternary phase diagram method and the solubility of blueberry anthocyanin nanoemulsions were taken into consideration. Deep purplish red, clear and transparent anthocyanin nanoemulsions were prepared by simple and cheap low-energy emulsification method according to the formula. Normal and high temperature test and high speed centrifugal test proved that the blueberry anthocyanin nanoemulsions had good stability. Guinea pig skin irritation test and sensitization test showed that there was no irritation and sensitization to guinea pig hair removal skin. In conclusion, the blueberry anthocyanin nanoemulsions prepared in this study have good stability at room temperature and are safe for guinea pig hair removal skin. It provides a reference for the application of blueberry anthocyanins in skin beauty and transdermal drug delivery.