Characterization of prebiotics and their synergistic activities with Lactobacillus probiotics for β-glucuronidase reduction

Abstract

The role of synbiotics for enriching health and well-being in addition to suppressing disease is gaining interest. Synergistic activities of four candidate prebiotics as exopolysaccharides (EPSs) derived from Lactobacillus fermentum TISTR 2514 (EPS-TISTR 2514), Pediococcus acidilactici TISTR 2612 (EPS-TISTR 2612), manno-oligosaccharides and rice syrup-oligosaccharides were characterized and evaluated for decreasing the risk of colorectal cancer (CRC). Results revealed that one or more candidate prebiotics stimulated the growth of Lactobacillus casei DSM 20011, Lactobacillus plantarum DSM 2648, and Lactobacillus rhamnosus DSM 20021 by at least two orders of magnitude higher than positive control (using FOS as carbon source) within 24 h in vitro. Simulated gastrointestinal (pH 1) and α-amylase (pH 7) resistance were tested. Results showed more than 75% remaining after incubation at 37 °C after 6 h for all treatments except rice syrup. L. plantarum DSM 2648 + manno-oligosaccharides (Tr.1), L. plantarum DSM 2648 + EPS-TISTR 2612 (Tr.2), L. rhamnosus DSM 20021 + rice syrup-oligosaccharides (Tr.3), L. casei DSM 20011 + EPS-TISTR 2514 (Tr.4), and L. rhamnosus DSM 20021 + EPS-TISTR 2514 (Tr.5) significantly decreased bacterial β-glucuronidase activity in rat feces demonstrating the potential of synergistic activity to reduce CRC risk. Synergistic activity of L. plantarum DSM 2648 + EPS-TISTR 2612 (Tr.2) showed the highest percentage reduction at 57.94 and 50.72% in male and female rats, respectively. Effectiveness of synbiotics in reducing the risk of CRC can be applied to future functional food development.