Synthesis and in vitro anti-HIV-1 activity of electrochemically reduced praseodymium salts of 12-tungstophosphate acid

Abstract

A praseodymium salt of 12-tungstophosphate acid, Pr[PW 12O 40], was synthesized using ion exchange and double decomposition method. Then its 2e and 4e electrochemically reduced heteropoly blues, PrH 2[PW vl10W v2O 40] and PrH 4[PW VIsW v4O 40], were synthesized using control-potential electrolysis reduction method. These compounds were characterized with FT-IR, UV-vis, polarography, ESR and XPS. The a-Keggin type structure was kept in the heteropoly blues PrH 2[PW vl10W v2O 40] and PrH 4[PW vlsW v4O 40] but the electronic density of heteropoly anion increased after electrochemically reduction. Heteropoly blues PrH 2[PW vl10W v2O 40] and PrH 4[PW vl8W v4O 40] showed relatively high in vitro anti-HIV-1 activity and relatively low cytotoxicity on MT-4 cells compared to some other anti-HIV-1 compounds. Because heteropoly blues possess higher negative electronic charges than their non-reduced parents, they strongly interact with HIV-1, MT-4 cells and macrophages and effectively suppress fusion, infection and proliferation of HIV-1. © 2010 Academic Journals Inc.