Identification of sea urchin gonads chemical compounds using thin-layer chromatography from Bokory island, Southeast Sulawesi

Abstract

Southeast Sulawesi is one area that has a diversity of potential marine biota as medicinal ingredients, including the sea urchin gonads. Empirically coastal communities have been hereditary, consuming sea urchin gonads as a source of high protein. Considering its potential as a raw material for medicines, it is necessary to conduct a series of tests to guarantee its safety and efficacy. This study aims to determine the chemical compounds of sea urchin gonad extract (Diadema setosum L) by Thin-Layer Chromatography (TLC). The research method uses a Pre-experimental design with a one-shot case study model. Identification of metabolites of sea urchin gonad extracts was carried out in two stages: phytochemical screening and identification by TLC method by UV-Vis. Samples were dried by the Freeze-drying method to get 335.11 g of dry samples and 58.06 g of extracts. The results of the test of the content of water-soluble and ethanol-soluble sea urchin extract were 5.73% and 3.72%. The results showed that in phytochemical screening, ethyl acetate extracts of the positive marine gonads contained alkaloids, saponins, and steroids. In addition, UV-vis 254 nm and 366 nm TLC methods showed alkaloids and saponins. The Rf values are 0.4 and 0.68, respectively.