AKTIVITAS ANTIHIPERURISEMIA KOMBINASI EKSTRAK DAUN KERSEN (MUNTINGIA CALABURA L.) DAN DAUN SIRSAK (ANNONA MURICATA L.) TERHADAP TIKUS PUTIH JANTAN

Abstract

Tanaman kersen (Muntingia calabura L.) dan sirsak (Annona muricata L.) memiliki banyak khasiat terhadap penyembuhan beberapa penyakit, diantaranya adalah antihiperurisemia yaitu gangguan metabolisme purin dimana penderita mengalami kenaikan kadar asam urat. Penelitian ini bertujuan untuk menguji efek antihiperurisemia kombinasi ekstrak daun kersen dengan sirsak pada tikus jantan secara oral. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 70%. Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan tikus putih jantan sebagai hewan uji .Data diolah berupa hasil uji ANOVA dan LSD (Least Significantly Difference) untuk membandingkan antara kelompok P0 (Kontrol normal), PI (Kontrol negatif), P2 (Allopurinol 9 mg/Kg BB), P3 (410 dan 3,5 mg/Kg BB), P4 (410 dan 7 mg/Kg BB), P5 (410 dan 10,5 mg/Kg BB), P6 (610 dan 3,5 mg/Kg BB), P7 (610 dan 7 mg/Kg BB), P8 (610 dan 10,5 mg/Kg BB), P9 (910 dan 3,5 mg/Kg BB), P10 (910 dan 7 mg/Kg BB), P11 (910 dan 10,5 mg/Kg BB). Pemberian bahan uji di mulai hari ke 8 sampai 14. Pada hari ke 15 diambil darah dengan cara disembelih untuk diukur kadar asam urat dengan menggunakan spektrofotometer UV-Vis.Pada pemeriksaan kadar asam urat, pemberian hati sapi dapat menaikkan kadar asam urat tikus jantan secara signifikan dan pemberian ekstrak daun  kersen dan daun sirsak dapat menurunkan kadar asam urat tikus jantan secara signifikan. Pemberian ekstrak daun kersen dan daun sirsak selama 7 hari pada dosis 910 dan 10,5 mg/Kg BB paling efektif menurunkan kadar asam urat pada tikus hiperurisemia.  Kersen (Muntingia calabura L.) and soursop (Annona muricata L.) have many properties to cure several diseases, including antihiperurisemia, which is a disorder of purine metabolism in which patients experience increased uric acid levels. This study aimed to examine the antihiperuricemia effect of a combination of kersen leaf extract and soursop in male rats orally. Extraction was carried out by maceration using 70% ethanol. This research is a laboratory experimental study using male white mice as test animals. Data were processed in the form of ANOVA and LSD (Least Significant Difference) test results to compare between groups P0 (normal control), PI (negative control), P2 (Allopurinol 9 mg/Kg BW), P3 (410 and 3.5 mg/Kg BW), P4 (410 and 7 mg/Kg BW), P5 (410 and 10.5 mg/Kg BW), P6 (610 and 3.5 mg/Kg BW), P7 (610 and 7 mg/Kg BW), P8 (610 and 10.5 mg/Kg BW), P9 (910 and 3.5 mg/Kg BW), P10 (910 and 7 mg/Kg BW) ), P11 (910 and 10.5 mg/Kg BW). Giving the test material starting on the 8th to 14th day. On the 15th day blood was taken by slaughtering to measure uric acid levels using a UV-Vis spectrophotometer. On examination of uric acid levels, the administration of beef liver can significantly increase uric acid levels in male rats. and the administration of kersen leaf extract and soursop leaves can significantly reduce uric acid levels in male rats. The administration of kersen leaf extract and soursop leaves for 7 days at doses of 910 and 10.5 mg/Kg BW most effectively reduced uric acid levels in hyperuricemic mice.   Keywords: Hyperuricemia, kersen leaves extract, soursop leaves extract