Evaluation of Substrates for Mass Culture of Phaeoacremonium parasiticum, the Fungus Responsible for Artificial Oleoresin Deposition in Agarwood Plant

Abstract

For establishing the favourable media for growth of Phaeoacremonium parasiticum, the fungus was inoculated in five different media (Potato Dextrose Broth (PDB), Host Extract (50%) + PDB (50%) (HE + PDB), and the solid media viz., Rice Bran (RB), Maize Meal (MM) and Wheat Bran (WB)) at pH of 6.5 and incubated at a temperature of 30˚C ± 1˚C. The solid media were added with water at the rate of 70 ml/100g of solid substrate. After 30 days of inoculation, conidial population of P. parasiticum in liquid media was lower compared to conidial population in solid media. In solid media, MM media showed the highest conidial population (9.56 log∙cfu/g) followed by WB (9.50 log∙cfu/g) and RB (9.38 log∙cfu/g). Hence, Maize Meal media (MM: Water = 100 g:70 ml) at pH of 6.5, incubation temperature: 30˚C ± 1˚C for 30 days would be the standard technique for mass production of P. parasiticum.