The evaluation of Two Zwitterionic Hydrophilic Interaction Liquid Chromatography Materials for the Rapid Separation of Methamphetamine and Muscimol Pharmaceuticals

Abstract

The investigation aimed to use two Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) columns to examine the retention behavior of methamphetamine and muscimol. The various spacer lengths between zwitterionic molecules in the stationary phases have been utilized to investigate methamphetamine and muscimol retention behavior. In hydrophilic interaction liquid chromatography (HILIC), the retention characteristic of these two drugs was examined using a mixture of sodium acetate buffer/acetonitrile as an eluent equipped with a UV detector. It has been established that hydrophilic and hydrophobic mechanisms primarily govern the separation of methamphetamine and muscimol medicines on two ZIC-HILIC columns. As previously stated, the ZIC-HILIC-1 column demonstrated a difference when compared to the ZIC-HILIC-5 columns. The combination of positively and negatively charged locations in a single molecule bonded to the surface of an adsorbent allows for distinct variations in methamphetamine and muscimol selectivity separation.