A simple and sensitive method for detecting avocado seed oil in various avocado oils

Abstract

The presence of avocado seed oil in various avocado oils was detected by thin-layer chromatography (TLC). An unsaponifiable compound, called Component-C, was identified in the avocado seed oil. This compound is unique to avocado seed and has a blue color when developed on the TLC plate. At least 9.2 μg of avocado seed unsaponifiables were needed at a single TLC spot to detect the unique blue color definitely. Admixtures of as little as 0.16% avocado seed oil in various avocado oils can be detected by the proposed procedure.