Vasopressin inhibits sarcolemmal ATP-sensitive K+ channels via V1 receptors activation in the guinea pig heart

Abstract

To examine the effect of vasopressin on the sarcolemmal ATP-sensitive K (KATP) channel, cell-attached, inside-out and open-cell-attached methods of patch clamp techniques were used in isolated guinea pig ventricular myocytes. Suppressing both glycolytic and oxidative ATP production attained KATP channel activation. In the cell-attached mode, vasopressin inhibited KATP channels in a concentration- dependent manner with an IC50 of 15.1±1.8 nmol/L. In the inside-out configuration, vasopressin failed to block KATP channels. In the cell-attached mode, manning compound (1μmol/L), a V1 receptor- selective antagonist, blocked the inhibitory action of vasopressin, although OPC-31260 (1μmol/L), a V2 receptor-selective antagonist could not affect the action of vasopressin. In addition, vasopressin lost its inhibitory action on KATP channels when the channel was activated by pinacidil, a K channel opener and in the open-cell-attached mode effected by streptolysin-O. Thus, the inhibitory action of vasopressin KATP channels may occur via V1 receptor related mechanism.