A turbidimetric method has been used for quantification of fractionated heparin (FH) in pharmaceutical dosage. The UV detection at two wavelengths (290 and 500 nm) showed a significant increase in sensitivity of the method, specificity, and linearity to range 5.0- 50.0 μg mL-1 and 50.0-200.0 μg mL-1, respectively (r > 0.99). At both wavelengths, the method was precise (inter-assay CV > 5.0%, and intra-assay CV > 3.0%), accurate (maximum deviation of ± 12%), and robust to the parameters evaluated. Turbidimetry proved to be easy, inexpensive and relatively fast. The results obtained attest to the reliability of the method.
CITATION STYLE
Marotta-Oliveira, S. S., & Marchetti, J. M. (2011). Determinação de heparina fracionada em preparaçõ es farmacêuticas utilizando turbidimetria. Quimica Nova, 34(6), 1063–1067. https://doi.org/10.1590/S0100-40422011000600025
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