Serological typing of Branhamella catarrhalis strains on the basis of lipopolysaccharide antigens

Abstract

A total of 302 strains of Branhamella catarrhalis from different parts of the world were serologically typed according to their lipopolysaccharide (LPS) antigenicity. For this purpose, an inhibition enzyme-linked immunosorbent assay was developed using the following reagents: antisera raised against whole bacterial suspensions for a panel of 16 serotype strains and LPS prepared from these strains by phenol extraction. Antisera were absorbed with whole bacterial suspensions of the B. catarrhalis strains to be tested. The residual activity of the sera against the homologous LPS was determined by means of an enzyme-linked immunosorbent assay, using microdilution plates coated with LPS. Strains which gave >90% reduction of activity were considered to carry the same LPS type as the serotype strain. It was shown that 93.4% of the strains tested carried one of three possible LPS types. LPS of B. catarrhalis are the rough type and have an apparent M(r) of 5,500, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.