Isolation and characterization of Alcaligenes sp. LS2T from poultry farm at Yogyakarta city and the growth ability in animal's urine medium

Abstract

This experiment aimed to identify isolate and to evaluate their capability for growing in ammonium and animal's urine high content medium. A nitrifying bacterium, strain LS2T was isolated from soil in the odorous region of poultry farms in Yogyakarta City due to the ability to encounter high dose of ammonium in the medium. Based on morphological, biochemical, and molecular identifications (16S rRNA sequence), strain LS2T was classified into the genus Alcaligenes, close to Alcaligenes faecalis ZJUTBX11, and it was identified as species Alcaligenes sp. LS2T. The 16S rRNA gene sequences were also compared using BLASTN, and strain LS2T showed high similarity to Alcaligenes (99 %). The cells of strain LS2T were rod-shaped [(0.5-1.0) μm × (0.5-2.6) μm], Gram-negative, and confirmed as oxides positive. Strain LS2T has the ability for growing in ammonium sulphate and animal's urine; it showed that strain LS2T could tolerate ammonium at high concentration. These results suggested the possibility of strain LS2T in deodorization plants for enhancing the efficiency of deodorization.