Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Abstract

Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified. Copyright © 2007, American Society for Microbiology. All Rights Reserved.