Potent pharmacophoric aminothiazole derivatives as FabH inhibitors for antibacterial activity: in vitro and in silico approach

Abstract

The present work reports the design, synthesis, characterization and antibacterial screening of novel aminothiazole derivatives as FabH inhibitor [β-ketoacyl-ACP synthase (KAS)] which plays major role in bacterial cell wall construction. The compound 5d had crystallized in monoclinic, P21/c space group which was determined by single-crystal X-ray crystallography. In vitro antibacterial activity studies were carried out on S. aureus (MCC 2043), E. faecalis (MTCC 2729), E. coli MTCC443 and C. violaceus (MCC 2216). Most of the compounds showed potent inhibition activity against Gram-negative bacteria than Gram-positive bacteria. Compound 5a showed the highest zone of inhibition of 16 mm and MIC value of 5.33 μM which is comparable to that of the standard antibiotic, streptomycin. This result was ably complimented by in silico studies where compound 5a exhibited high affinity, strong binding energy and docking score of 6.214 kcal mol−1. The most potent compounds were nonhemolytic and nontoxic to mammalian cells.