Spectrophotometric determination of naproxen using chitosan capped silver nanoparticles in pharmaceutical formulation

Abstract

Naproxen (NP) is a non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for the treatment of acute to chronic pain. The aim of this work was to develop a new, simple spectrophotometric method for determining the concentration of naproxen in pharmaceutical formulations by chitosan capped silver nanoparticles. The morphology and structure of the chitosan capped silver nanoparticles were determined by ultraviolet spectroscopy (UV), Fourier transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), dynamic light scattering (DLS) and zeta potential (ZP) under optimal conditions. The results showed that, synthesized chitosan capped silver nanoparticles were approximately with particle size of 100 nm poly dispersity index (PDI) 0.385 and strong anionic (-24.8mV) zeta potential at acidic condition. It was found that chitosan as a chiral selector was able to detect naproxen at optimal conditions. The method was successful to determine naproxen in drug tablets formulation. A relative standard deviation of 1.0% was determined for the analysis of naproxen in real samples. Validation of this method, including limit of detection and limit of quantification were accurately confirmed according to ICH instructions. Based on this method, the limit of detection (LOD) and the limit of quantification (LOQ) of naproxen were calculated 0.022 and 0.066 molL-1, respectively. Analysis of statistical data, the reproducibility and accuracy of this method demonstrated that the use of this novel method is valuable and practical for determination of naproxen in pharmaceutical formulations.