Molecular species identification of red shrimp (Crustacea: Decapoda: Barbouriidae) from Tanjung Sanjangan (Tolitoli, Central Sulawesi) through 16S rRNA mitochondrial gene

Abstract

Red shrimp is one of the endemic aquatic animals inhabited at Tanjung Sanjangan, Tolitoli, Central Sulawesi. However, the molecular identification of red shrimp has not been investigated. Accurate identification of the red shrimp is important for conservation in its habitat. Therefore, the objective of this study was to identify the red shrimp in Tanjung Sanjangan, Tolitoli, Central Sulawesi based on the 16S mitochondrial gene. The method applied in this study was a PCR method with universal primers 16Sar and 16Sbr. The species identification was analyzed using Nucleotide BLAST. Intraspecific and interspecific analysis was done using DnaSP. The Neighbor-Joining method with Kimura 2 Parameter model was used to reconstruct phylogenetic tree. Additional 16S rRNA sequences taken from GenBank was used for a comparative purpose and outgroup. Nucleotide BLAST analysis revealed that all of the 10 samples of red shrimps collected from Tanjung Sanjangan were verified as Parhippolyte uveae. The NJ tree was also supported the 10 samples of red shrimp clustered with Parhippolyte uveae (accession number KF178886). For the 533 bp sequence examined in the red shrimp, two haplotypes were detected with one variable site which was considered as parsimony informative. Haplotype diversity and nucleotide diversity were 0.556 and 0.001 respectively. This occurrence indicated low genetic variation of the red shrimps collected from Tanjung Sanjangan, Tolitoli, Central Sulawesi.