DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF KETOPROFEN AND THIOCOLCHICOSIDE IN SOLID ORAL DOSAGE FORM

Abstract

A newer, simple, rapid, accurate, precise and cost-effective Q-Absorbance ratio method has been developed for the simultaneous estimation of Ketoprofen and Thiocolchicoside in solid oral dosage form and validated as per International Conference of Harmonisation guidelines. Absorbance ratio method was developed using two wavelengths which are 275 nm and 251.5 nm respectively. Methanol was used as a solvent. The linearity was found in the range of 5-25 μg/ml and 4-20 μg/ml for Ketoprofen and Thiocolchicoside respectively. The linearity coefficient was found to be 0.995 and 0.990 for Ketoprofen and 0.986 and 0.990 for Thiocolchicoside at 251.5 nm 275 nm respectively. % RSD studies were found to be less than ± 2. The Limit of Detection and Limit of Quantitation were found to be and 16.58 μg/ml and 50.25 μg/ml for Ketoprofen and 13.33 μg/ml and 40.40 μg/ml for Thiocolchicoside respectively. The % recovery was found to be 99.84% and 99.20% for Ketoprofen and Thiocolchicoside respectively. The developed method is simple, precise and accurate and it can be used for routine analysis of both drugs in their combined solid oral dosage form