Abstract
The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter. The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.
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CITATION STYLE
Schaffert, L., Jacob, L., Schneiker-Bekel, S., Persicke, M., März, C., Rückert, C., … Kalinowski, J. (2020). pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Microbiology Resource Announcements, 9(39). https://doi.org/10.1128/mra.00596-20
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