The effects of temephos, permethrin and malathion selection on the fitness and fecundity of Aedes aegypti

Abstract

The recent scale-up of insecticide use has led to the rapid spread of insecticide resistance (IR) in mosquito populations across the world. Previous work has suggested that IR mechanisms could influence mosquito life-history traits, leading to alterations in fitness and key physiological functions. This study investigates to what extent mosquito fitness may be affected in a colony of Aedes aegypti after selection with temephos, permethrin or malathion insecticides. We measured immature development, sex ratio, adult longevity, energetic reserves under different rearing conditions and time points, ingested bloodmeal volume, mosquito size, male and female reproductive fitness and flight capability in the unexposed offspring of the three selected strains and unselected strain. We found that insecticide selection does have an impact on mosquito fitness traits in both male and female mosquitoes, with our temephos-exposed strain showing the highest immature development rates, improved adult survival, larger females under crowded rearing and increased sperm number in males. In contrast, this strain showed the poorest reproductive success, demonstrating that insecticide selection leads to trade-offs in life-history traits, which have the potential to either enhance or limit disease transmission potential.