Synthesis of Agnps of Momordica charantia Leaf Extract, Characterization and Antimicrobial Activity

Abstract

The physicochemical and optoelectronic properties of metallic nanoparticles are strongly dependent on the size and size-distribution of the nanoparticles. In this study the silver nanoparticles were synthesized from leaf extract of Momordica charantia at room temperature as well as stirred at 60°C and their antibacterial activity was studied by Disc Diffusion Method. The effects of different leaf extract concentrations, metal ions concentration, reaction times and reaction temperatures on the synthesis of silver nanoparticles were evaluated. The Nanoparticles were characterized with UV-Visible, XRD, SEM, and FTIR. The UV-Vis spectra showed that the Surface Plasmon Resonance peak of silver colloids synthesized from M.charantia leaf extract were observed at 404 nm and 424 nm for stirred at 60°C and room temperature condition. X-ray diffraction (XRD) analysis confirmed that the nanoparticles were crystalline in nature with Face Centered Cubic structure. Scanning Electron Microscopy (SEM) analysis showed that silver nanoparticles were spherical in shape with an average size of 20-50 nm. The FTIR measurement was carried out to identify the possible functional groups responsible for efficient stabilization of silver nanoparticles. The inhibitory activity of prepared silver nanoparticles were tested against the pathogen Staphylococcus aureus