Problem-solving Test: DNA manipulation with alkaline phosphatase and polynucleotide kinase

Abstract

Terms to be familiar with before you start to solve the test: 3′- and 5′-ends of DNA fragments, plasmids, restriction endonucleases, agarose gel electrophoresis, linear and circular DNA, superhelical DNA, blunt and sticky ends, DNA ligase, nuclease, [γ-32P] ATP, and radioactive labeling. © 2014 The International Union of Biochemistry and Molecular Biology.