Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

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Abstract

Abstract: Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption.

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Makarova, J. A., Fomicheva, K. A., Osipyants, A. I., Shkurnikov, M. Y., Pokryshchenko, A. A., Tonevitsky, E. A., & Vechorko, V. I. (2021). Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics. Applied Biochemistry and Microbiology, 57(8), 845–850. https://doi.org/10.1134/S0003683821080032

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