Occurrence of Pasteurellaceae and Neisseriaceae bacteria in the pharyngeal and respiratory tract of dogs and cats - Short communication

Abstract

The occurrence ofmembers of the Pasteurellaceae and Neisseriaceae families was studied in dogs and cats. A total of 110nasal and pharyngeal swab samples from47dogs and 8 catswere collected. Most of the strainswere identified by 16S rDNA sequencing, except Frederiksenia canicola and Pasteurella multocida where speciesspecific polymerase chain reactions were applied. Themost frequently isolated species was F. canicola, which occurred only in dogs, mainly in the pharyngeal cavity. The second commonest bacterium, P. multocida was found in both types of samples and in both hosts. Other species from the family Pasteurellaceae, such as Haemophilus haemoglobinophilus, Pasteurella canis and P. dagmatis, were detected only in dogs. All isolated species belonging to the family Neisseriaceae, mainly representing Neisseria weaveri, were found only in the pharyngeal cavity. Neisseria weaveri and N. zoodegmatis could be detected in both hosts. Neisseria dumasiana and N. canis were isolated from dogs,whileN. shayeganii only froma cat. For phylogenetic analysis, rpoB gene sequencingwas performed,where the strainswere onmonophyletic branches and clearly separated fromeach other. In this study, recently described species such as F. canicola, N. shayeganii and N. dumasiana were detected that had never been isolated in Hungary before.