Inhibition of transforming growth factor-β, hypoxia-inducible factor-1α and vascular endothelial growth factor reduced late rectal injury induced by irradiation

Abstract

Tumor hypoxia and angiogenesis associated with malignant progression have been studied widely. The efficacy of angiogenesis inhibition combined with radiotherapy has been demonstrated in cancer treat- ment. Here, we studied the effect of hypoxia and angiogenesis inhibition on radiation-induced late rectal injury. The rectum of C57BL/6N mice was irradiated locally with a single dose of 25 Gy. Radiation- induced histological changes were examined at 90 days after irradiation by hematoxylin-eosin (H.E.) staining and azan staining. Pimonidazole was administered and its distribution was assayed by immunohis- tochemistry staining. Expression of transforming growth factor β1 (TGF-β1), hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) was assessed on the fibrotic region using real- time PCR and immunohistochemistry. In addition, the effects of TGF-β, VEGF and HIF-1α on radiation- induced injury were investigated by the administration of neutralizing antibody of TGF-β, antibody of VEGF or YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) which was developed as an agent for inhibiting HIF-1 expression after irradiation respectively. Fibrosis and uptake of pimonidazole were found 90 days after irradiation. The expression of TGF-β1, HIF-1α and VEGF significantly increased with the formation of fibrosis induced by irradiation compared with unirradiated controls. In addition, treatment of neutralizing antibody of TGF-β, antibody of VEGF or YC-1 reduced the development of radiation-induced injury. Our results suggested that radiation-induced hypoxia may play an important role in late rectal injury. Although the inhibition of HIF-1α and VEGF reduced the radiation induced late injury, the precise mechanism is still unclear.