Determination of Residual Diaveridine and Sulfaquinoxaline in Hen's Egg, Chicken Plasma and Tissues by High-Performance Liquid Chromatography

Abstract

A high-performance liquid chromatographic method for the determination of diaveridine (Dv) and sulfaquinoxaline (Sq) in hen's egg, chicken plasma, liver, kidney, heart, spleen, muscle, gizzard, skin and fat was developed using a C18 reversed-phase column and simple mobile phases. The pretreatments of Dv and Sq in such biological materials were classified into six groups. Detection limits were 0.02–0.04 ppm for Dv and 0.01–0.03 ppm for Sq. Average recoveries of Dv and Sq from egg and plasma were above 77%, and those from all other tissues ranged from 53 to 78%. The method was applied for residue analysis of Dv and Sq in egg yolk and albumen. © 1981, The Pharmaceutical Society of Japan. All rights reserved.