Nocardioides caricicola sp. nov., an endophytic bacterium isolated from a halophyte, Carex scabrifolia Steud.

Abstract

A Gram-staining-positive, coccoid to rod-shaped bacterium, designated strain YC6903T, was isolated from a halophytic plant (Carex scabrifolia Steud.) collected from sand dunes at. Namhae Island, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC6903T grew optimally at 30 °C and at pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC6903 T belongs to the genus Nocardioides in the family Nocardioidaceae. Strain YC6903T was related most closely to Nocardioides pyridinolyticus OS4T (97.0% 16S rRNA gene sequence similarity), Nocardioides dokdonensis FR1436T (96.6%), Nocardioides aquiterrae GW-9T (96.6%) and Nocardioides hankookensis DS-30T (96.6%). The cell-wall peptidoglycan contained LL-diaminopimelic acid and MK-8(H4) was the major respiratory quinone. The mean (±SD) level of DNA-DNA relatedness between strain YC6903T and N. pyridinolyticus OS4T was 53.5±5.5%. The predominant cellular fatty acid of strain YC6903T was iso-C16 : 0 (28.9%). The DNA G+C content was 71.7 mol%. Phenotypic, phylogenetic and chemotaxonomic data indicated that strain YC6903T represents a novel species of the genus Nocardioides, for which the name Nocardioides caricicola sp. nov. is proposed. The type strain is YC6903T (=KACC 13778T =DSM 22177 T). © 2011 IUMS.