Southern hybridization of radiolabeled probes to nucleic acids immobilized on membranes

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Abstract

In this protocol, restriction fragments that have been transferred to a membrane by Southern blotting are hybridized to a labeled probe. Methods for stripping the probe from the membrane are also included.

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Green, M. R., & Sambrook, J. (2021). Southern hybridization of radiolabeled probes to nucleic acids immobilized on membranes. Cold Spring Harbor Protocols, 6(7), 280–289. https://doi.org/10.1101/pdb.prot100495

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