Analytical Detection of Triterpenoids Present in the Hydroalcoholic Extract of Ipomoea Aquatica Forssk. in South India

Abstract

Introduction: Qualitative analysis will help in the detection of phytoconstituents present in the herbal source accurately. The preliminary phytochemical screening and the thin layer chromatographic analysis are found more simple and sensitive and selective techniques in this way.Aim and Objective:The research was aimed to reveal the secondary metabolites like triterpenoids by using chemical tests and TLC methods. Methods: The tests for detecting triterpenoids were using Salkowski’ sreagent and Sulphur powder tests. The TLC parameters were set silica gel G as adsorbent, Toluene: Ethyl acetate in the ratio of 9.3:0.7v/v as mobile phase, UV light of longer wavelength at 365nm as detection wavelength and Rf value as qualitative respect. Results and Discussion:The phytochemical tests were shown positive results for triterpenoids. The TLC analysis stated that the presence of nearly five different fluorescence spots with Rf values of 0.06, 0.11, 0.23, 0.36 and 0.59 respectively. Conclusion:Hence, revealing new class of lipophilliccomponents will assist to improve herbal drug products in the global market. This study could be used in research laboratories for detecting similar type of compounds using TLC analysis. Definitely this will give the good for opportunity for isolating out many therapeutically acting compounds.