Metastatic events of MDA-MB-231 cells induced by angiogenic factors VEGF or MYA1 are inhibited by Tinospora cordifolia hexane fraction (tchf)

Abstract

ngiogenic growth factors VEGF or MTA1 mediated proliferation, migration and invasion play pivotal role in the process of metastasis. In this paper we have described the preparation of hexane fraction from Tinospora cordifolia (Tchf) and have shown that Tchf has an antiangiogenic activity. HUVEC tube formation and Rat cornea angiogenesis assays were used for validation of antiangiogenic activity of Tchf. Our results on VEGF or MTA1 induced proliferation, migration and invasion of MDA-MB-231 cells show that Tchf and SB202190, a MAP kinase inhibitor effectively inhibits these metastatic processes. Both angiogenic factors VEGF or MTA1 could induce expression of VEGF gene in MDA-MB-231 cells, Tchf repressed VEGF gene expression induced either by VEGF or MTA1. These results clearly indicate for the first time that Tchf is a potent inhibitor of metastatic events which are synergistically trigged by VEGF and MTA1