Analytical Platforms for Mass Spectrometry-Based Proteomics

Abstract

Mass spectrometry-based proteomics platforms have been widely used as 'proteome sequencers' to characterize the proteomes of a wide range of organisms. The work-flow generally involves multiple steps of sample preparation, peptide purification/concentration/pre-fractionation, and nanoLC/MS/MS measurement. This review focuses on our contributions to the technical development of current proteomics platforms, and includes a consideration of the limitations of these systems, together with the prospects for developing superior new-generation proteome sequencers.