Bioremoval capacity of three heavy metals by some microalgae species (Egyptian isolates)

Abstract

Three fresh water microalgal isolates [Phormidium ambiguum (Cyanobacterium), Pseudochlorococcum typicum and Scenedesmus quadricauda var quadrispina (Chlorophyta)] were tested for tolerance and removal of mercury (Hg2+), lead (Pb2+) and cadmium (Cd2+) in aqueous solutions as a single metal species at conc. 5-100mg/L under controled laboratory conditions. The obtained results showed that Hg2+ was the most toxic of the three metal ions to the test algae even at low concentration (, 20 mg/L). While lower concentration of Pb2+ and Cd2+ (5-20 mg/L) enhanced the algal growth (chlorophyll a and protein), elevated concentrations (40-100mg/L) were inhibitory to the growth. The results also revealed that Ph. ambiguum was the most sensitive alga to the three metal ions even at lower concentrations (5 and 10mg/L) while P. typicum and S. quadricauda were more tolerant to high metal concentrations up to 100mg/L. The bioremoval of heavy metal ions (Hg2+, Pb2+ and Cd2+) by P. typicum from aqueous solution showed that the highest percentage of metal bioremoval occurred in the first 30 min of contact recording 97% (Hg2+), 86% (Cd2+) and 70% (Pb2+). Transmission electron microscopy (TEM) was used to study the interaction between heavy metal ions and P. typicum cells. At ultrastructural level, an electron dense layers were detected on the algal cell surfaces when exposed to Cd, Hg, and Pb. At the same time, dark spherical electron dense bodies were accumulated in the vacuoles of the algal cells exposed to Pb. Excessive accumulation of starch around the pyrenoids were recorded as well as deteriorations of the algal cell organelles exposed to the three metal ions. © 2012 Landes Bioscience.