Dose-Dependent Toxicological and Histological Effects of Chloramphenicol in Nude Mice

Abstract

Chloramphenicol is a widely used broad-spectrum antibiotic in both human and veterinary medicine. Although it is successful in treating a number of bacterial diseases, concerns have been raised about its negative effects. To examine the toxicological and histological consequences of chloramphenicol in vivo, this study used a naked mouse model. After chloramphenicol injection, the health of the cells was assessed using histological examinations of the liver, kidney, and heart tissues. The cell viability of the liver, kidney, and heart tissues decreased by 39.02%, 43.04%, and 36.25%, respectively, at the highest dose (100mg/kg body weight). The liver, kidneys, and heart, respectively, each had an average damage score of 3.5, 4.0, and 3.6 at the maximum dosage, according to histological findings, which indicated serious organ damage. Because of the kidney's role in excreting drugs, the toxic effects seen were notably more pronounced in this organ. These findings highlight the potential risks of using chloramphenicol, particularly at high dosages, and the need for more research to understand the mechanisms underlying chloramphenicol-induced toxicity.