Biological and physico-chemical properties of the infectious bursal disease virus (ibdv)

Abstract

Some biological properties of an egg-adapted strain of IBDV, PV1, have been studied. By imimunofluorescent staining the strain is serologically identical to another egg-adapted strain, MU2, and to three pathogenic strains of IBDV. All five strains do not react with antisera prepared against known avian viruses of various groups, including Reoviruses. The egg-adapted strain PV1 replicates in chick embryo fibroblasts and in RK13 cells, with the formation of plaques. The PVl strain, highly neuropathogenic for newborn mice, is not neuropathogenic for newborn chicks. Various physico-chemical characteristics of IBDV have been studied in comparison to Crawley Reovirus, namely resistance to 5-iodo-2-deoxyuridine, heat-resistance, stability over a wide pH range, resistance to photodynamic inactivation and UV irradiation, sensitivity to Actinomycin D. As Reoviruses, IBDV is a naked RNA Virus, sensitive to Actinomycin D. It is more resistant than Reoviruses to heat, UV irradiation and photodynamic inactivation. All these findings are discussed in relationship to the taxonomic position of IBDV. Cytological observations are reported on cells infected with IBDV and the growth curve is described. Les AA. ont etudie les proprietes biologiques d'une souche adaptee à I’embryori du poulet de IBDV, PV1. La souche est serologiquement identique a une autre souche adaptee a I'embryon du poulet, MU2, et a trdis souches pathogenes de IBDV., Toutes les cinq souches ne reagissent pas avec les serums anti prepares contre les virus aviaires connus, y compris les Reovirus. Les souches PV1 se multi multiplient dans les fibroblastes D'embryon de poulets et dans les cellules RK 13 avec formation de plaques. La souche PV1, tres neuropathogene pour les souris nouveau-nees, ne l'est point pour les poulets nouveau-nes. I Is ont aussi etudie les differentes caracteristiques physico-chimiques de IBDV en comparaison au Reovirus Crawley c'est a dire resistance a 5-iiodo-2’deoxyuridine, resistance a la chaleur, stabilite en milieu aoide et aloaline, resistance a rinacfivation photodynamique et a I'irradiation ultraviolette, sensibilite a rActinomioine D. Comme les Reovirus, I'lBDV est un virus RNA nu, sensible a I'Actinomicine D. II est plus resistant que les Reovirus a la chaleur, a I'irradiation ultraviolette dt a I'inactivation photodynamique. Ces proprietes du virus ont ete discutees en relation à la position taxonomique de I'lBDV. Les AA. ont decriit ausSi les observations cytologiques sur les cellules infectees avec IBDV et la courbe de multiplication. © 1973, Taylor & Francis Group, LLC. All rights reserved.