Environment-sensitive fluorescent turn-on probes targeting hydrophobic ligand-binding domains for selective protein detection

Abstract

Bind and shine: An approach for the selective detection of both enzymes and non-enzymatic proteins using an environment-sensitive fluorescent turn-on probe is described (see scheme). This approach targets the hydrophobic ligand-binding domain of the target protein to trigger the fluorescence turn-on and was shown to be specific for the targeted protein. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.