Quantitative determination of tocopherols by high-speed liquid chromatography

Abstract

A high-speed liquid chromatographic method for the determination of α-,β-,γ- and δ-tocopherols using a spectrophotometer or a spectrofluorometer, is described. The best results were obtained using a 2.3 mm i.d.×500mm normal phase partition column, JASCOPACK WC-03-500 and a spectrofluorometer as a specific detector. A mixture of diisopropyl ether and n-hexane (2:98) was used as the mobile phase. A flow rate was set at 0.8ml/min. A mixture of pure α-,β-,γ- and δ-tocopherols or natural tocopherols in vegetable oils were separately determined by this method. © 1975, Center for Academic Publications Japan. All rights reserved.