INHIBISI ENZIM α-GLUKOSIDASE OLEH SENYAWA FLAVONOID DAUN KELOR (Moringa oleifera) IN SILICO DAN IN VITRO

Abstract

Moringa leaves flavonoid have potential to inhibit α-glucosidase but they have low bioavailability, so they aremade in nanoparticles. It is also not known which specific flavonoid compounds from Moringa leaves have thepotential to inhibit α-glucosidase. This research aimed to determine the inhibition potential of α-glucosidase bythe moringa leaves flavonoid in silico through molecular docking and determine the inhibitory activity of α-glucosidase by moringa leaves flavonoid in extracts and nanoparticles in vitro. Moringa leaves flavonoid havepotential to be a competitive inhibiton of α-glucosidase with the highest to lowest inhibitory potential arecryptochlorogenic acid, quercetin-3-O-beta-D-glucopyranoside, quercetin-3-glucoside, kaempferol-3-O-a-ramnoside, kaempferol-3-O-glucoside, epicathechins, catechins, quercetin, kaempferol, glucomoringinisothiocyanate. Cryptochlorogenic acid has the best potential with ΔG and Ki values -8.5 kcal/mol and 0.5788μM. Inhibition α-glucosidase moringa leaves flavonoid in extract and nanoparticles respectively are classified asinactive (IC 50 = 5.84x10 3 ppm) and active (IC 50 = 1.59x10 1 ppm) in vitro, so nanoparticles can increase theinhibitory activity.