Esterification of Glucose by Coconut Oil Fatty Acids Using Immobilized CandidarugosaLipaseE.C.3.1.1.3. inCa-alginate Matrix

Abstract

In this study, the synthesis of fatty acid-carbohydrate esters used glucose (G) and coconut oil fatty acid (FA). The esterification reaction was carried out enzymatically using Candida rugosalipase in organic solvent (n-hexane). Lipase was immobilized on Ca-alginate gel beads withthe optimum condition for immobilization was1 % of Na-alginate, resulting 61.66% immobilization yield. The optimum conditions for esterification using immobilized lipase in Ca-alginate were at 35 o C, the molar ratio 1:60 (G/FA), 16 hours incubation time and 0 g molecular sieve. Characterization of synthesized product with FT-IR showed that product exhibit the absorbtion of ester functional group at 1737 cm-1 , but with thegreater OH groupabsorption peakat3344.57cm-1 indicatedthat the degree ofesterificationwas still low. The synthesized product was then examined by simple emulsion test and was proved to be an emulsifier. Based on theemulsion test, it was known that theproducts ofthe reactionwereglucoseestersthat have propertiesampiphilicas anemulsifiersystem ofoil-in-water emulsion(o/w).