The effect of adipose-derived stem cell-cultured media on oxazolone treated atopic dermatitis-like murine model

Abstract

Background: A stem cell is an undifferentiated cell that has the potential for self-renewal and differentiation. Adiposederived stem cells (ADSCs) have advantages in accessibility and abundance compared to other kinds of stem cells and produce many growth factors and hormones. Objective: We investigated whether ADSC cultured media could be used as a therapy for atopic dermatitis. Methods: ADSC cultured media was topically applied twice daily for 5 days to oxazolone-treated atopic dermatitis-like hairless mice. Results: Topical application of ADSC cultured media improved the epidermal permeability barrier and keratinocyte differentiation, and restored the predominant Th2 phenotype when compared to vehicle. ADSC cultured mediatreated epidermis also showed an increase in the expression of antimicrobial peptides cathelin-related antimicrobial peptide, mouse beta-defensein 3. Conclusion: Topical ADSC cultured media could be useful in the treatment of atopic dermatitis.