In vitro properties of chitosan nanoparticles induce apoptosis in human lymphoma SUDHL-4 cell line

Abstract

In this study, the possible mechanisms were investigated with chitosan nanoparticles using sodium tripoly-phosphate and effects on human lymphoma SUDHL-4 in vitro. It was characterized by XRD, FTIR, TGA, particle Size, zeta potential, SEM & TEM. Different techniques such as cell proliferation, ultra structure changes, DNA fragmentation, phase distribution of cell cycle, MTT assay, MMP, agarose gel electrophoresis of DNA, flow cytometry and electron microscopy were used with treatment of different concentrations of CH-NPs (25, 50, 75, 100 µg/ml) at different time periods. Electron microscopy study revealed that the chitosan nanoparticles showed 78 nm particle size which is a high surface charge as 52 mV. Inhibition of chitosan nanoparticles after 48h treatment was marked in cell proliferation of SUDHL-4 with an IC50 value of 5 µg/ml. Electron microscopy showed typical necrotic cell morphology after treatment of chitosan nanoparticles.