Development of semi-nested pcr for detection of 16s rrna gene of mycoplasma hyosynoviae

Abstract

In Thailand, some breeders in pig farms have had osteoarthritis lesions for which M. hyosynoviae might be one of the causative agents. Therefore, the semi-nested polymerase chain reaction assay was developed for detection of Mycoplasma hyosynoviae in organs of pigs in Thailand using three oligonucleotide primers specific to 16S rRNA gene of M. hyosynoviae. The detection limit of purified DNA was 10 femtogram per reaction and of the simulated lung sample was 103 CFU per gram of sample. Preliminary study for tonsil-carrier state of M. hyosynoviae in slaughtered pigs revealed the presence of M. hyosynoviae in 5 out of 10 farms. Thus, the semi-nested PCR is a useful tool for presumptive screening of M. hyosynoviae presenting in pig herds.