Transcriptome reveals the molecular mechanism of chaetoviridin A inhibiting the spore germination of Verticillium dahliae

Abstract

The pathogen Verticillium dahliae causes Verticillium wilt in a number of crops, including cotton Verticillium wilt. Chaetoviridin A, a secondary metabolite of Chaetomium globosum, significantly inhibits the growth of V. dahliae. Spore germination is a major part of the disease cycle. However, the molecular mechanism of chaetoviridin A inhibiting spore germination of V. dahliae is unknown. In this work, we found that chaetoviridin A significantly inhibited spore germination of V. dahliae. Transcriptome analysis showed that DEGs were enriched in linolenic acid metabolism, alpha-Linolenic acid metabolism, Arachidonic acid metabolism and Purine metabolism pathways at 1 h, which were related to cell membrane. At 3 h, DEGs were enriched in the pathways of galactose metabolism, diterpenoid biosynthesis, cysteine and methionine metabolism, and starch and sucrose metabolism, which were mainly related to amino acid metabolism and sugar metabolism. Several genes related to glucose metabolism were identified, mainly including Glucagon endo-1,3-alpha-glucosidase agn1, glucoamylase, maltose O-acetyltransferase, and beta-galactosidase. Stress resistance gene PAL, detoxification gene P450 2C31 and ABA receptor were also down- regulated. These genes may be related to spore germination. These results provide a theoretical basis for chaetoviridin A to control fungal diseases.